Frozen sectioning method is the most commonly used sectioning technique in histochemistry, especially enzyme histochemistry, fixed or unfixed tissue samples can be frozen section, fresh tissue frozen sections for tissue intracellular enzyme preservation is also a commonly used sectioning method in immunocytochemical research, can better preserve a variety of antigens, especially the antigenicity of surface antigens. 

1. Preparation

Clean up the test bench and instruments

Turn on the cryomicrotome and pre-reduce the cryomicrotome to the required temperature (-15~-20 °C)

Prepare the processed slides, blades, glue and medicines 

2. Clamp the frozen tissue block on the microtome holder, start the rough advance and withdrawal button, turn the knob, and smooth the tissue.

3. Adjust the thickness to be cut, according to different tissues, in principle, it is a cell-intensive thin cut, and the fiber multicellular thin can be slightly thick cut, generally between 5 ~ 10μm.

4. Adjust the anti-coil plate. The key to making frozen slices lies in the adjustment of the anti-roll plate, which requires the operator to be careful and accurately adjust it well and adjust it to the appropriate position. When slicing, cut out a complete, smooth slice. When the cut tissue adheres to a clean slide, gently apply a gentle force in one direction to avoid wrinkles during the tissue spreading process, ensure the integrity of the tissue structure and the beauty of the section.