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The production process of frozen sections

2024 / 06 / 06

Frozen sectioning method is the most commonly used sectioning technique in histochemistry, especially enzyme histochemistry, fixed or unfixed tissue samples can be frozen section, fresh tissue frozen sections for tissue intracellular enzyme preservation is also a commonly used sectioning method in immunocytochemical research, can better preserve a variety of antigens, especially the antigenicity of surface antigens.  1. PreparationClean up the test bench and instrumentsTurn on the cryomicrotome and pre-reduce the cryomicrotome to the required temperature (-15~-20 °C)Prepare the processed slides, blades, glue and medicines  2. Clamp the frozen tissue block on the microtome holder, start the rough advance and withdrawal button, turn the knob, and smooth the tissue. 3. Adjust the thickness to be cut, according to different tissues, in principle, it is a cell-intensive thin cut, and the fiber multicellular thin can be slightly thick cut, generally between 5 ~ 10μm. 4. Adjust the anti-coil plate. The key to making frozen slices lies in the adjustment of the anti-roll plate, which requires the operator to be careful and accurately adjust it well and adjust it to the appropriate position. When slicing, cut out a complete, smooth slice. When the cut tissue adheres to a clean slide, gently apply a gentle force in one direction to avoid wrinkles during the tissue spreading process, ensure the integrity of the tissue structure and the beauty of the section.

Overview of frozen slicer equipment? The supplier of fully automatic paraffin slicer will take you to understand

2016 / 09 / 06

A frozen slicer refers to an instrument that can rapidly produce arrangement slices for observation by achieving a certain hardness at low temperatures. The frozen slicer consists of slicing equipment and a refrigeration system. A complete frozen slicing system is generally composed of a host (freezer box, sample head, quick freezing table, etc.), a knife holder, and blades; Some frozen slicers also have vacuum equipment, which is used to suck up the waste inside the machine and assist in flattening the slices. The frozen slicer is an equipment produced in Germany, with a slicing scale of 1-500 μ m. The function of the frozen slicer is that it is suitable for directly slicing arranged in a frozen state.In the frozen slicing machine, the embedded sections are arranged to be frozen until they are firm, and before freezing, no chemical treatment or heating process is arranged, greatly reducing the production time. Meanwhile, due to the fact that the arrangement does not require processes such as dehydration, visualization, and waxing, it is more suitable for fat, nerve arrangement, and some chemical preparation methods, and is widely used as a rapid sectioning method in clinical diagnosis.The fundamental operation of a frozen slicer:1. Turn on the power 2 hours before use and set the temperature to -25 ℃;2. Place a specimen of appropriate size and thickness on a mushroom shaped cup, add frozen wax, and clip it into the fixed slot of the slicer;3. Prepare the glass slides, select the thickness of the slices, and swing the handle to make the slices;4. Carefully adhere the sliced pieces onto the glass sheet;5. After use, close the power supply, clean the chamber and wipe off any moisture, and keep a record of the use.6. To allow water vapor to evaporate, please do not immediately close the sliding door of the slicer cover. It should be closed every other day. If you need specific instructions, please borrow the instruction manual.The above content is presented to you by the supplier of fully automatic paraffin slicer equipment.
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